With his goal against Athletic Club on Saturday, Karim Benzema moved on to 119 LaLiga goals and equalled Amancio's tally for the club in the process.
Both men now sit level on 119 league goals for Real, ninth in the all-time rankings.
Benzema's first half goal was his eighth in 15 games against the Bilbao outfit, with three of those strikes coming at Estadio San Mames.
The opener also moved the Frenchman on to 16 goals for the campaign, with eight of those coming in LaLiga, and makes him the second top scorer for Real this year behind Cristiano Ronaldo, who is on 26.
Real Madrid's highest LaLiga goalscorers
Cristiano Ronaldo 280
Di Stéfano 216
Hugo Sánchez 164